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  3. Nav1.5 (D9J7S) Rabbit Monoclonal Antibody (BSA and Azide Free)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Nav1.5 (D9J7S) Rabbit Monoclonal Antibody (BSA and Azide Free) #63973

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 230-280
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #14421. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #14421

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Nav1.5 (D9J7S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Nav1.5 protein. This antibody also recognizes bands at 190 and 135 kDa that are likely degradation products of Nav1.5.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Nav1.5 protein.

    Background

    Voltage gated sodium channels are composed of a large alpha subunit and auxiliary beta subunits. The alpha subunit has 4 homologous domains, with each domain containing 6 transmembrane segments. These segments function as the voltage sensor and sodium permeable pore. Upon change of membrane potential, the sodium channel is activated, which allows sodium ions to flow through (1,2). When associated with beta subunits or other accessory proteins, the alpha subunit is regulated at the level of cell surface expression, kinetics, and voltage dependence (3,4).

    There are 9 mammalian alpha subunits, named Nav1.1-Nav1.9 (5). These alpha subunits differ in tissue specificity and biophysical functions (6,7). Seven of these subunits are essential for the initiation and propagation of action potentials in the central and peripheral nervous system while Nav1.4 and Nav1.5 are mainly expressed in skeletal muscle and cardiac muscle (8,9). Mutations in these alpha channel subunits have been identified in patients with epilepsy, seizure, ataxia, sensitivity to pain, and cardiomyopathy (reviewed in 10).
    Nav1.5 is a type V alpha subunit. It is expressed primarily in cardiac muscle and is accountable for the initiation and propagation of the cardiac action potentials (11). Mutations in the SCN5A gene encoding Nav1.5 have been associated with arrhythmic cardiac diseases such as Brugada syndrome, long QT syndrome 3, and Progressive familial heart block 1A (PFHB1A) (12-15). Nav1.5 is also expressed in jejunal circular smooth muscle (HJCSM) cells. Research studies identified loss of function of Nav1.5 in 2% of patients with irritable bowel syndrome (9,16). Nav1.5 has multiple splice variants, several of which are expressed in the brain (17-18).
    1. Catterall, W.A. (2000) Neuron 26, 13-25.
    2. Yu, F.H. and Catterall, W.A. (2003) Genome Biol 4, 207.
    3. Isom, L.L. et al. (1994) Neuron 12, 1183-94.
    4. Yu, F.H. et al. (2003) J Neurosci 23, 7577-85.
    5. Goldin, A.L. et al. (2000) Neuron 28, 365-8.
    6. Plummer, N.W. and Meisler, M.H. (1999) Genomics 57, 323-31.
    7. Goldin, A.L. (2001) Annu Rev Physiol 63, 871-94.
    8. George, A.L. et al. (1992) Ann Neurol 31, 131-7.
    9. Ou, Y. et al. (2002) Neurogastroenterol Motil 14, 477-86.
    10. Meisler, M.H. and Kearney, J.A. (2005) J Clin Invest 115, 2010-7.
    11. Gellens, M.E. et al. (1992) Proc Natl Acad Sci U S A 89, 554-8.
    12. Wang, Q. et al. (1995) Cell 80, 805-11.
    13. Chen, Q. et al. (1998) Nature 392, 293-6.
    14. Schott, J.J. et al. (1999) Nat Genet 23, 20-1.
    15. Tan, H.L. et al. (2001) Nature 409, 1043-7.
    16. Beyder, A. et al. (2014) Gastroenterology 146, 1659-68.
    17. Wang, J. et al. (2009) Neurosci Res 64, 339-47.
    18. Ren, C.T. et al. (2012) Mol Cell Biochem 365, 139-48.

    Alternate Names

    cardiac sodium channel alpha subunit; cardiac tetrodotoxin-insensitive voltage-dependent sodium channel alpha subunit; CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; Nav1.5; PFHB1; SCN5A; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type 5 subunit alpha; sodium channel protein type V alpha subunit; Sodium channel protein type V subunit alpha; sodium channel, voltage-gated, type V, alpha subunit; sodium voltage-gated channel alpha subunit 5; SSS1; VF1; Voltage-gated sodium channel subunit alpha Nav1.5

    Database Links

    UniProt ID: Q14524


    Entrez-Gene Id: 6331


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