p21 Waf1/Cip1 Antibody

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WB
IP

Inquiry Info. # 64016

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Product Specifications

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	21
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

p21 Waf1/Cip1 Antibody recognizes endogenous levels of total mouse p21 Waf1/Cip1 protein. This antibody does not recognize endogenous levels of human or rat p21 Waf1/Cip1 protein, but does weakly recognize overexpressed human p21 Waf1/Cip1 protein.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro122 of mouse p21 Waf1/Cip1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

Alternate Names

CAP; CAP20; CDK; CDK-interacting protein 1; CDKI; Cdkn; Cdkn1; Cdkn1a; CDN1A; Cip1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); mda; mda6; Melanoma differentiation-associated protein; p21; p21<WAF1/CIP1>; p21C; p21Cip1; p21W; p21WAF; SDI1; Waf; Waf1

Database Links

UniProt ID: P39689

Entrez-Gene Id: 12575

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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p21 Waf1/Cip1 Antibody #64016