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CaMKII (pan) (D11A10) Rabbit Monoclonal Antibody (BSA and Azide Free) #65517

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60, 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #4436. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #4436

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    CaMKII (pan) (D11A10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total CaMKII protein. The peptide sequence used as the antigen is 100% conserved between CaMKII-alpha, gamma and delta, and 88% conserved in CaMKII-beta.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Val184 of human CaMKII-alpha.

    Background

    CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

    Alternate Names

    calcium/calmodulin dependent protein kinase II alpha; calcium/calmodulin dependent protein kinase II beta; calcium/calmodulin dependent protein kinase II delta; calcium/calmodulin dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; Calcium/calmodulin-dependent protein kinase type II subunit delta; Calcium/calmodulin-dependent protein kinase type II subunit gamma; calmodulin-dependent kinase II; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II delta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM kinase II subunit delta; CaM kinase II subunit gamma; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaM-kinase II delta chain; CAM2; CAMK; CAMK-II; CaMK-II alpha subunit; CaMK-II delta subunit; CaMK-II subunit alpha; CaMK-II subunit beta; CaMK-II subunit delta; CaMK-II subunit gamma; CAMK2; CaMK2-beta; CaMK2-delta; CAMK2A; CAMK2B; CAMK2D; CAMK2G; CAMKA; CAMKB; CAMKD; CAMKG; CaMKII; CaMKIIalpha; CaMKIIbeta; CaMKIINalpha; FLJ16043; KCC2A; KCC2B; KCC2D; KCC2G; KIAA0968; MGC26678; MGC29528; MGC44911; MRD53; MRD54; MRD59; MRT63; proline rich calmodulin-dependent protein kinase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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