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MEIS1 (E9V1D) Rabbit Monoclonal Antibody #67260

Filter:
  • WB
  • IP
  • ChIP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 52, 62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MEIS1 (E9V1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total MEIS1 protein. This antibody does not cross-react with MEIS2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro51 of human MEIS1 protein.

    Background

    Hox, Pbx, and Meis are families of transcription factors that bind DNA via their homeodomains. Members from each family form heterodimers to give rise to complexes with unique DNA binding specificities. Homeodomain-containing proteins are frequently involved in normal developmental processes but can also be associated with tumorigenic states (1). MEIS proteins belong to the three amino acid loop extension (TALE) homeobox-containing transcription factor family. MEIS1 has been associated with leukemogenesis and neuroblastoma (2,3), while MEIS2 is known to play an important role in the transcriptional program that is induced in normal pancreatic development (4) and cardiogenesis (5).

    Alternate Names

    Homeobox protein Meis1; leukemogenic homolog protein; Meis homeobox 1; MEIS1; Meis1, myeloid ecotropic viral integration site 1 homolog; myeloid ecotropic viral integration site 1; WUGSC:H_NH0444B04.1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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