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Render Timestamp:
5/20/2026, 7:28:47 AM EDT
5/20/2026, 11:28:47 AM UTC
Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2026-05-13 07:53:47.067
Product last modified at: 2026-05-13T08:55:36.756Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TXNIP (D5F3E) Rabbit Monoclonal Antibody (Biotinylated) #67280

Filter:
  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the biotinylated version of the unconjugated TXNIP (D5F3E) Rabbit Monoclonal Antibody #14715 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.
    MW (kDa) 55

    Product Usage Information

    Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

    Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TXNIP (D5F3E) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total TXNIP protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val337 of human TXNIP protein.

    Background

    The ubiquitously expressed thioredoxin-interacting protein (TXNIP) binds and inhibits thioredoxin to regulate cellular redox state (1-3). Research studies demonstrate that hyperglycemia induces TXNIP expression and increases cellular oxidative stress (1). In addition, these studies show that TXNIP reduces glucose uptake directly by binding the glucose transporter Glut1 to stimulate receptor internalization or indirectly by reducing Glut1 mRNA levels (3). Additional studies indicate that TXNIP plays a role in the regulation of insulin mRNA transcription (4). Microarray analyses indicate that TXNIP acts downstream of PPARγ and is a putative tumor suppressor that may control thyroid cancer cell progression (5). In addition, the TXNIP protein may be a potential therapeutic target for the treatment of type 2 diabetes and some disorders related to ER-stress (6).

    Alternate Names

    ARRDC6; EST01027; HHCPA78; THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; TXNIP; upregulated by 1,25-dihydroxyvitamin D-3; VDUP1; Vitamin D3 up-regulated protein 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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