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SARS-CoV-2 ORF7a Antibody #67750

Filter:
  • WB

    Product Specifications

    REACTIVITY Vir
    SENSITIVITY Endogenous
    MW (kDa) 9-14
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 ORF7a Antibody recognizes endogenous levels of total SARS-CoV-2 ORF7a protein.

    Species Reactivity:

    Virus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val74 of SARS-CoV-2 ORF7a protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    SARS-CoV-2 ORF7a is a type-I transmembrane protein with an amino-terminal immunoglobulin (IgG)-like domain involved in immune modulation and a short cytosolic tail that contains an endoplasmic reticulum (ER) retention motif that mediates trafficking to the ER-Golgi intermediate complex (ERGIC) (1). Studies of ORF7a in SARS-CoV identified roles in apoptosis and cell cycle progression (2-4). Regulation of apoptosis has been attributed to an association with Bcl-xL, a key pro-survival Bcl-2 family member (5). Studies in both SARS-CoV and SARS-CoV-2 have shown that ORF7a can promote viral release through inhibition of BST2 (6,7). There are also reports that SARS-CoV-2 contributes to immune regulation as part of the viral life cycle. It can inhibit type I interferon signaling through inhibition of STAT2 phosphorylation (8). However, it can also lead to activation of NF-κB and induce the expression of inflammatory cytokines (9). ORF7a has also been shown to inhibit late stages of autophagy by reducing lysosomal acidity (10).

    Alternate Names

    Accessory protein 7a; NS7A_SARS2; ORF7a protein; Protein 7a; Protein U122; Protein X4; SARS-CoV-2 accessory protein 7a; Severe acute respiratory syndrome coronavirus 2 ORF7a protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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