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MAP4K3 (E9L1J) Rabbit Monoclonal Antibody #69387

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 101
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAP4K3 (E9L1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAP4K3 protein. A band of unknown identity is detected at 48 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His404 of human MAP4K3 protein.

    Background

    The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3).

    Alternate Names

    4833416M01Rik; 4833416M07Rik; 9530052P13Rik; germinal center kinase-like kinase-like; Germinal center kinase-related protein kinase; GLK; KHS2; M4K3; Map4k3; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; mitogen activated protein kinase kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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