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c-IAP1 (E6R2S) Rabbit Monoclonal Antibody #70008

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  • WB
  • IP

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    c-IAP1 (E6R2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total c-IAP1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse c-IAP1 protein.

    Background

    The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

    Alternate Names

    Api1; Api2; apoptosis inhibitor 2; AW146227; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing 3; Baculoviral IAP repeat-containing protein 2; Birc2; Birc3; C-IAP1; C330006D17Rik; Cellular inhibitor of apoptosis 1; cIA; cIAP1; cIAP2; HIAP1; HIAP2; IAP-2; IAP1; Iap2; Inhibitor of apoptosis protein 2; mcI; mcIAP1; mIAP-2; MIAP1; mIAP2; MIH; MIHB; MIHC; RING-type E3 ubiquitin transferase BIRC2; RNF48

    For Research Use Only. Not for Use in Diagnostic Procedures.
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