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GOT2 Antibody #71692

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 41
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GOT2 Antibody recognizes endogenous levels of total GOT2 protein. This antibody does not cross-react with GOT1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp54 of human GOT2 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Glutamate oxaloacetate transaminase 2 (GOT2), a mitochondrial enzyme, catalyzes the conversion of oxaloacetate to aspartate. Studies show that the metabolite pyridoxal phosphate (PLP) is selectively required by acute myeloid leukemia. PLP-dependent GOT2 selectively sustains leukemic cell proliferation. Depletion of GOT2 suppresses the proliferation of acute myeloid leukemia cells (1). In addition, mouse hematopoietic stem cells are dependent on cell-autonomous aspartate synthesis. Got2 deletion leads to decreased aspartate levels and reduced hematopoietic stem cell function (2). Furthermore, GOT2-dependent aspartate synthesis is also necessary for protein and collagen biosynthesis in chondrocytes and is critical for chondrocyte proliferation and growth (3).

    Alternate Names

    AATM; aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; aspartate transaminase 2; FABP-1; FABPpm; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; glutamic-oxaloacetic transaminase 2; glutamic-oxaloacetic transaminase 2, mitochondrial; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); GOT2; KAT4; KATIV; KYAT4; Kynurenine aminotransferase 4; kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; mAspAT; mitAAT; Plasma membrane-associated fatty acid-binding protein; Transaminase A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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