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PathScan® RP MAP2A/MAP2B Sandwich ELISA Kit #72831

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    Product Specifications

    REACTIVITY M
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP MAP2A/MAP2B Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MAP2A/MAP2B protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with MAP2A/MAP2B in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of MAP2A/MAP2B protein. Learn more about your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP MAP2A/MAP2B Sandwich ELISA Kit detects endogenous levels of MAP2A/MAP2B protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Mouse

    Background

    Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).

    Alternate Names

    G1-397-34; MAP; MAP-2; Map2; Microtubule-associated protein 2; Mta; Mtap-2; Mtap2; OTTMUSP00000017688; OTTMUSP00000017689; OTTMUSP00000017691; repro4

    For Research Use Only. Not for Use in Diagnostic Procedures.
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