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NUFIP1 (E7E4L) Rabbit Monoclonal Antibody #74030

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NUFIP1 (E7E4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUFIP1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein.

    Background

    NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2).

    Alternate Names

    bA540M5.1; FMR1-interacting protein NUFIP1; nuclear FMR1 interacting protein 1; Nuclear FMRP-interacting protein 1; nuclear fragile X mental retardation protein interacting protein 1; nuclear fragile X mental retardation protein-interacting protein 1; Nuclear fragile X mental retardation-interacting protein 1; NUFIP; NUFIP1; NUFIP1, FMR1 interacting protein 1; NUFP1; Rsa1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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