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Cystatin-A (F2W9D) Rabbit Monoclonal Antibody #75912

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  • WB
  • IHC
  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 11
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cystatin-A (F2W9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystatin-A protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human cystatin-A protein.

    Background

    Cystatin-A (CST-A), also known as cystatin SN or cystatin-1, is a member of the cystatin superfamily of proteins. Its primary function is the reversible and competitive inhibition of cysteine proteases, particularly lysosomal cathepsins (e.g., cathepsin B, H, and L). This inhibitory action is crucial for regulating cellular protein turnover, tissue remodeling, and maintaining cellular homeostasis (1). Our understanding of the role that cystatin-A plays in disease is incomplete. Cystatin-A is reported to be upregulated in several solid tumors, and elevated expression has been associated with enhanced cell proliferation, migration, invasion, and poor prognosis (2-4), suggesting that it might act as an oncogenic driver. However, some studies reporting decreased cystatin-A expression suggest it may play a protective role in certain tumor types (5,6).

    Alternate Names

    AREI; CSTA; cystatin A; cystatin A (stefin A); cystatin AS; Cystatin-A; Cystatin-A, N-terminally processed; Cystatin-AS; CYTA; PSS4; stefin A; Stefin-A; STF1; STFA

    For Research Use Only. Not for Use in Diagnostic Procedures.
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