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CLEC4A (F2X3I) Rabbit Monoclonal Antibody #76213

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  • WB
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30-40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Live) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CLEC4A (F2X3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLEC4A protein. This antibody detects a 65 kDa and a 100 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn200 of human CLEC4A protein.

    Background

    C-type lectin domain family 4 member A (CLEC4A), also referred to as dendritic cell (DC) immunoreceptor (DCIR), is a type II membrane glycoprotein, primarily expressed on myeloid-derived cells including monocytes, DCs, macrophages, and neutrophils (1-4). CLEC4A contains an ITIM motif in the cytoplasmic domain, which is involved in regulating immune response (1,2,5). CLEC4A provides anti-inflammatory signaling through the recruitment of phosphatases SHP-1 and SHP-2 (3-5). Insufficient CLEC4A function might cause autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) (4,5).

    Alternate Names

    C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6; C-type lectin DDB27; C-type lectin domain family 4 member A; C-type lectin superfamily member 6; CD367; CLC4A; CLEC4A; CLECSF6; DCIR; DDB27; Dendritic cell immunoreceptor; HDCGC13P; Lectin-like immunoreceptor; LLIR

    For Research Use Only. Not for Use in Diagnostic Procedures.
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