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GRB7 (E3W4G) Rabbit Monoclonal Antibody #76518

Filter:
  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 62
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GRB7 (E3W4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRB7 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro87 of human GRB7 protein.

    Background

    GRB7 belongs to the GRB7 family of adaptor proteins that mediate cell surface and cytosolic tyrosine kinases to downstream signaling pathways (1-3). The C-terminal SH2 domain of GRB7 has been shown to be important for its interaction with various upstream molecules such as ERBB receptor tyrosine kinases, FAK, EphB1, and SHC. The protein has an RA, CAM-BD, and PH domain located at the central region (GM region) of its sequences to mediate downstream Ras, PI3K/Akt, ERK, and Calmodulin signaling (3,4). Overexpression of GRB7 promotes cancer proliferation and migration, and disruption of GRB7 function inhibits its effect on cancer growth, invasion, and metastasis (3-5).

    Alternate Names

    B47; Epidermal growth factor receptor GRB-7; GRB7; GRB7 adapter protein; growth factor receptor bound protein 7; Growth factor receptor-bound protein 7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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