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  3. PLEKHM1 (E9S3Q) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PLEKHM1 (E9S3Q) Rabbit Monoclonal Antibody #77092

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PLEKHM1 (E9S3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total PLEKHM1 protein. This antibody does not cross-react with Rubicon protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human PLEKHM1 protein.

    Background

    Pleckstrin homology domain-containing protein family member 1 (PLEKHM1) is a multivalent endolysosomal adaptor protein that contains an N-terminal RUN domain, two internal PH domains, and a C-terminal C1 domain/zinc-finger-like motif. Research studies have shown that PLEKHM1 plays a role in bone resorption by regulating the acidification of endosomal vesicles of osteoclasts. Loss-of-function mutations in PLEKHM1 promote osteoclast failure and impair bone resorption, which leads to osteopetrosis (1-3). Recent studies have shown that PLEKHM1 serves as a nexus for different trafficking pathways of the endolysosomal network by serving as the platform for the recruitment of Rab7, HOPS complex, and LC3/GABARAP family members. Indeed, loss of PLEKHM1 impairs the lysosomal degradation of cell-surface receptors and autophagic clearance of protein aggregates (4,5).

    Alternate Names

    162 kDa adapter protein; AP162; B2; KIAA0356; OPTA3; OPTB6; PH domain-containing family M member 1; PKHM1; pleckstrin homology and RUN domain containing M1; pleckstrin homology domain containing, family M (with RUN domain) member 1; Pleckstrin homology domain-containing family M member 1; PLEKHM1

    Database Links

    UniProt ID: Q9Y4G2


    Entrez-Gene Id: 9842


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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