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MKL1/MRTF-A (E3N7W) Rabbit Monoclonal Antibody #77098

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 145
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MKL1/MRTF-A (E3N7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total MKL1/MRTF-A protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MKL1/MRTF-A protein.

    Background

    The megakaryoblastic leukemia proteins 1 and 2 (MKL1, MKL2) are myocardin-related transcription factors (MRTF-A, MRTF-B) that serve as actin-regulated transcription coactivators for the serum response factor (SRF). Interaction between G-actin and MKL proteins retains the coactivator within the cytoplasm of resting cells. Activated Rho-A promotes F-actin assembly and a reduction of the G-actin pool in serum-stimulated cells. This results in the accumulation of MKL proteins in the nucleus, where the coactivator associates with the SRF to activate target gene transcription and mediate multiple cellular processes (1-4). A number of other signaling pathways, including the TGFβ, BMP, and PDGF pathways, also make use of MKL-mediated activation of target gene transcription (5-9). Chromosomal translocations involving the genes encoding MKL1 and MKL2 have been identified in several cases of acute megakaryoblastic leukemia and chondroid lipoma (10-12).

    Alternate Names

    basic, SAP and coiled-coil domain; BSAC; KIAA1438; MAL; megakaryoblastic leukemia (translocation) 1; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MKL; MKL/myocardin-like protein 1; MKL1; MRTF-A; MRTFA; myocardin related transcription factor A; Myocardin-related transcription factor A; RBM15/MKL1 fusion; RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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