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SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody

SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody #77860

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	60
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SASH3/SLY protein.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro53 of human SASH3/SLY protein.

Background

Encoded by an X-linked gene, sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY), is a putative lymphoid-specific signaling adaptor (1). One of the first clues that SASH3 plays a role in regulating the biology of lymphoid cells arose from research studies demonstrating that SASH3 is modified by phosphorylation subsequent to T cell receptor (TCR) engagement (2). Furthermore, the presence of SAM and SH3 domains strongly suggests that SASH3/SLY participates in protein-protein interactions to regulate immune cell effector functions downstream of antigen receptor engagement. Indeed, naturally occurring loss-of-function mutations have been identified in human SASH3/SLY that blunt activation of multiple key signaling nodes following TCR engagement, leading to an impairment of T cell proliferation and survival (3).

Alternate Names

753P9; CXorf9; HACS2; SAM and SH3 domain containing 3; SAM and SH3 domain-containing protein 3; SASH3; SH3 protein expressed in lymphocytes homolog; SH3D6C; SLY

Database Links

UniProt ID: O75995

Entrez-Gene Id: 54440

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.

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