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SHMT1 (D3B3J) Rabbit mAb #80715

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Inquiry Info. # 80715

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SHMT1 (D3B3J) Rabbit mAb recognizes endogenous levels of total SHMT1 protein. This antibody does not cross-react with SHMT2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu187 of human SHMT1 protein.

    Background

    Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).

    Alternate Names

    14 kDa protein; CSHMT; cytoplasmic serine hydroxymethyltransferase; GLYC; Glycine hydroxymethyltransferase; MGC15229; MGC24556; serine hydroxymethyltransferase 1; serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; SHMT1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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