Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RNASEH2A (F5D8B) Rabbit Monoclonal Antibody #82457

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RNASEH2A (F5D8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total RNASEH2A protein. This antibody does not cross-react with RNASEH2B protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu289 of human RNASEH2A protein.

    Background

    The RNase H2 complex forms as a heterotrimer composed of RNASEH2A, RNASEH2B, and RNASEH2C. RNASEH2A is the catalytic component and is well conserved across species, while RNASEH2B and RNASEH2C are quite divergent (1). The RNase H2 complex is responsible for resolving DNA/RNA hybrids, known as R-loops, which can occur during DNA replication and repair (1,2). The C-terminus of RNASEH2B is bound to PCNA, which then guides the complex to nuclear replication foci to resolve misincorporated ribonucleotides (2). Replicative stress leads to the increased expression of RNase H2 subunits, thereby reducing replication fork stalling in response to chemotherapeutics (3). Mutations in RNase H2 subunits cause Aicardi-Goutieres syndrome (AGS), a rare form of encephalopathy characterized by an increased innate immune response triggered by unresolved R-loops, mimicking a viral infection (4-6). RNASEH2B is frequently lost along with RB1 in advanced prostate cancer, which sensitizes cells to PARP and ATR inhibitors (7,8).

    Alternate Names

    AGS4; Aicardi-Goutieres syndrome 4 protein; JUNB; Ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease H2, subunit A; Ribonuclease HI large subunit; Ribonuclease HI subunit A; ribonuclease HI, large subunit; RNase H(35); RNase H2 subunit A; RNase HI large subunit; RNASEH2A; RNASEHI; RNH2A; RNHIA; RNHL; THSD8

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.