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Vitronectin (F7A8D) Rabbit Monoclonal Antibody #82821

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 78
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Vitronectin (F7A8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total vitronectin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile465 of human vitronectin protein.

    Background

    Vitronectin (VTN) is a multidomain adhesive glycoprotein synthesized mostly in the liver and secreted to blood plasma and extracellular matrix (ECM) (1,2). The protein has an N-terminal SMB fragment mediating binding to PAI-1 and UPAR, followed by an RGD region mediating its interaction with integrins, and C-terminal hemopexin-like domains mediating its interaction with various ECM components such as collagens, tenascins, and plasminogen (2,3). VTN has been implicated as a regulator of many diverse physiological processes, including coagulation, complement-dependent immune response, cell attachment and spreading, angiogenesis, hemostasis, and vascular remodeling (4-6). Increased VTN expression promotes cancer cell migration, adhesion, and metastasis in neuroblastoma, ovarian cancer, and breast cancer cells (7-9).

    Alternate Names

    complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; Somatomedin-B; V75; Vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN; VTNC

    For Research Use Only. Not for Use in Diagnostic Procedures.
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