Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

hnRNP E2/PCBP2 (D1S5E) Rabbit Monoclonal Antibody #83017

Filter:
  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 35-45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    hnRNP E2/PCBP2 (D1S5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP E2/PCBP2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu243 of human hnRNP E2/PCBP2 protein.

    Background

    PCBP2 (also known as hnRNP E2) is an RNA-binding protein that interacts in a sequence-specific fashion with single-stranded poly (rC). Through their poly(rC)-binding ability, PCBPs regulate mRNA stability and translation (1,2). PCBP2 is an iron chaperone; it delivers iron to ferritin for storage and mediates metalation of certain iron-containing proteins (3-5). PCBP2 interacts with the iron importer DMT1 (divalent metal transporter 1) and the iron exporter FPN1 (ferroportin 1) and regulates their activities (6,7). PCBP2 is induced by viral infection and targets MAVS for polyubiquitination and degradation (8-10). Recent reports demonstrate that it is involved in Hippo signaling, miRNA processing, immune suppression, and cancer (11-15) .

    Alternate Names

    Alpha-CP2; epididymis secretory sperm binding protein; Heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP-E2; HNRNPE2; HNRPE2; MGC110998; PCBP2; poly(rC) binding protein 2; Poly(rC)-binding protein 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.