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PTPN18 (D7B9) Rabbit Monoclonal Antibody #8311

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 48
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PTPN18 (D7B9) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPN18 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala401 of human PTPN18 protein.

    Background

    PTPN18 is a member of the PEST family of nonreceptor protein tyrosine phosphatases, a group that also includes PTP-PEST and PEP (1-3). Members of this protein family contain an N-terminal catalytic domain and a noncatalytic C-terminal domain with a PEST motif that mediates protein-protein interactions (4). PTPN18 was first identified in hematopoietic stem cells (HSCs), where it was suggested to play a role in HSC differentiation (1-3). Subsequently, PTPN18 was shown to bind to c-Src kinase, forming a protein complex that functions to inhibit Src-family kinase signaling (4,5). More recently, PTPN18 expression has been shown to correlate with HER2 receptor expression in breast cancer cell lines (6,7), where it has been suggested to repress HER2 signaling (7).

    Alternate Names

    BDP1; Brain-derived phosphatase; protein tyrosine phosphatase non-receptor type 18; protein tyrosine phosphatase, non-receptor type 18 (brain-derived); PTN18; PTP-HSCF; PTPN18; Tyrosine-protein phosphatase non-receptor type 18

    For Research Use Only. Not for Use in Diagnostic Procedures.
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