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Rab32 (F6E5T) Rabbit Monoclonal Antibody #84334

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  • WB
  • IP

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab32 (F6E5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab32 protein. This antibody detects a 55 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys208 of mouse Rab32 protein.

    Background

    Rab32 is a small GTPase best known for its role in regulating membrane trafficking, including endosomal pathway regulation and Golgi apparatus structure and localization (1,2). Rab32 is an essential component of a host defense pathway for defending against intracellular pathogens, helping to link lysosomes and bacteria-containing phagosomes (3,4). Rab32 also recruits IRG1, an enzyme that produces the antimicrobial metabolite itaconate, to pathogen-containing vacuoles (5). Altered Rab32 function is implicated in the pathogenesis of Parkinson's disease. The RAB32 Ser71Arg mutation, which results in increased activation of LRRK2 and reduced colocalization of Rab32 and PINK1, is associated with development of Parkinson's disease (6).

    Alternate Names

    2810011A17Rik; AU022057; Rab32; RAB32, member RAS oncogene family; Ras-related protein Rab-32

    For Research Use Only. Not for Use in Diagnostic Procedures.
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