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  3. SPAK (F7T1K) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SPAK (F7T1K) Rabbit Monoclonal Antibody #84671

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 65
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    Immunofluorescence (Frozen) 1:400 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPAK (F7T1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPAK protein. Non-specific punctate cytoplasmic staining was observed in human kidney and staining of intercalated discs of human heart was observed by immunohistochemistry.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu429 of human SPAK protein.

    Background

    SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family of serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase, indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3,4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knockdown of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).

    Alternate Names

    DCHT; DKFZp686K05124; PASK; proline-alanine-rich STE20-related kinase; serine threonine kinase 39 (STE20/SPS1 homolog, yeast); serine/threonine kinase 39; Serine/threonine-protein kinase 39; small intestine SPAK-like kinase; SPAK; Ste-20-related kinase; Ste20-like protein kinase; STE20/SPS1 homolog; STE20/SPS1-related proline-alanine-rich protein kinase; STK39; STLK3

    Database Links

    UniProt ID: Q9UEW8


    Entrez-Gene Id: 27347


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