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RASSF1 (E7T6O) Rabbit Monoclonal Antibody #86026

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RASSF1 (E7T6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total RASSF1 protein. This antibody is predicted to detect the following RASSF1 isoforms: 1A, 1D, 1E, 1F, and 1G. It is not predicted to detect isoforms 1B, 1C, or 1H, as these isoforms lack the amino acid sequence corresponding to the antigen.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg50 of human RASSF1 protein.

    Background

    Ras association domain-containing protein 1 (RASSF1) is a member of the RASSF protein family (RASSF1-10), scaffold proteins whose members are unified by the presence of a Ras association (RA) domain that gives them structural similarity to Ras effector proteins (1). The RASSF1 gene is located in a genomic region exhibiting loss of heterozygosity in a more than 90% of small cell lung cancers, and up to 50% of non-small cell lung cancers (2), strongly implicating the RASSF1 gene as a tumor suppressor. Moreover, RASSF1 expression in tumor cells has been shown to be frequently suppressed by promoter hypermethylation, further suggesting a tumor suppressor function (3-5). Although multiple isoforms (RASSF1A-H) have been described, generated by alternative splicing and/or promoter usage, RASSF1A and RASSF1C appear to be the most widely expressed in normal tissues, with RASSF1A expression lost most frequently in tumor cells (6). Notably, RASSF1A has been identified as an effector protein in the Hippo signaling pathway, where it promotes association between MST1/2 and LATS1/2, leading to suppressive phosphorylation of the transcriptional co-activators YAP and TAZ (7).

    Alternate Names

    123F2; cardiac-specific ras association domain family 1 protein; NORE2A; pancreas-specific ras association domain family 1 protein; Ras association (RalGDS/AF-6) domain family 1; Ras association (RalGDS/AF-6) domain family member 1; Ras association domain family member 1; Ras association domain family protein 1; Ras association domain-containing protein 1; RASF1; RASSF1; RASSF1A; RDA32; REH3P21; tumor suppressor protein RDA32; WUGSC:H_LUCA12.5

    For Research Use Only. Not for Use in Diagnostic Procedures.
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