Phospho-YAP (Ser61) (E4Q8S) Rabbit Monoclonal Antibody #86312
- WB
- IP
- IHC
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
YAP function is influenced by cellular metabolic status. This was recognized after observing that AMPK, a master regulator of cell metabolism, directly phosphorylates YAP at multiple sites (e.g., Ser61, Ser94) during energy stress or nutritional deprivation (11-13). Phosphorylation of Ser61 by AMPK did not increase 14-3-3 binding or affect YAP subcellular localization, but nevertheless resulted in transcriptional repression of YAP target genes (12).
- Sudol, M. (1994) Oncogene 9, 2145-52.
- Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
- Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
- Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
- Yagi, R. et al. (1999) EMBO J 18, 2551-62.
- Dong, J. et al. (2007) Cell 130, 1120-33.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Yu, F.X. et al. (2012) Cell 150, 780-91.
- Zhao, B. et al. (2010) Genes Dev 24, 72-85.
- Mo, J.S. et al. (2015) Nat Cell Biol 17, 500-10.
- Wang, W. et al. (2015) Nat Cell Biol 17, 490-9.
- Hariharan, I.K. (2015) Nat Cell Biol 17, 362-3.
Alternate Names
65 kDa Yes-associated protein; COB1; Protein yorkie homolog; Transcriptional coactivator YAP1; YAP; YAP1; YAP2; YAP65; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; Yes1 associated transcriptional regulator; YKI; Yorkie homolog
Limited Uses
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