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RRM1 (D12F12) Rabbit Monoclonal Antibody #8637

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  • IHC
  • IF
  • F

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 90
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100
    Immunofluorescence (Immunocytochemistry) 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #63356.

    Protocol

    Specificity / Sensitivity

    RRM1 (D12F12) Rabbit Monoclonal Antibody recognizes endogenous levels of total RRM1 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RRM1 protein.

    Background

    Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).

    Alternate Names

    R1; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; ribonucleotide reductase catalytic subunit M1; Ribonucleotide reductase large subunit; ribonucleotide reductase M1; ribonucleotide reductase M1 polypeptide; ribonucleotide reductase, large subunit; ribonucleotide reductase, R1 subunit; RIR1; RR1; RRM1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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