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Phospho-PFAS (Thr619) Antibody

Phospho-PFAS (Thr619) Antibody #87933

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	145
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-PFAS (Thr619) Antibody recognizes endogenous levels of PFAS protein only when phosphorylated at Thr619.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr619 of human PFAS protein. Antibodies are purified by peptide affinity chromatography.

Background

Phosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the “purinosome,” which is involved in de novo purine synthesis (1). Research studies have shown that activation of the MAPK/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the de novo generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3).

Alternate Names

FGAM synthase; FGAMS; FGAR amidotransferase; FGAR-AT; FGARAT; Formylglycinamide ribonucleotide amidotransferase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase; GATD8; IKZF1/PFAS fusion; KIAA0361; PFAS; Phosphoribosylformylglycinamidine synthase; PUR4; PURL

Database Links

UniProt ID: O15067

Entrez-Gene Id: 5198

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For Research Use Only. Not for Use in Diagnostic Procedures.

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