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MHC Class I (EMR8-5) Mouse Monoclonal Antibody #88274

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  • WB
  • IHC
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30-50
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #28149.

    Protocol

    Specificity / Sensitivity

    MHC Class I (EMR8-5) Mouse Monoclonal Antibody detects HLA-A, HLA-B, HLA-C, and HLA-E. This antibody does not cross-react with HLA-G.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human HLA-A*2402 extracellular domain recombinant protein.

    Background

    The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8+ T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4+ T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2).

    Alternate Names

    AS; B-4901; D6S204; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA-A; HLA-B; HLA-C; HLA-C antigen; HLA-Cw; HLA-JY3; HLAA; HLAB; HLAB iso9; HLAC; HLC-C; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leukocyte antigen class I-A; leukocyte antigen class I-B; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, A; major histocompatibility complex, class I, B; major histocompatibility complex, class I, C; MHC; MHC class 1 antigen; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; PSORS1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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