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CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy5.5® Conjugate) #90088

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Inquiry Info. # 90088

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    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rat IgG2a kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.25μg of antibody per test.
    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:80
    Flow Cytometry (Live) 1:80

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total mouse CD62L protein.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).

    Alternate Names

    AI528707; CD62; CD62 antigen-like family member L; CD62L; L-sel; L-selectin; LAM-1; LECAM; LECAM-1; LECAM1; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; Ly-2; Ly-22; Ly-m2; Ly-m22; Ly22; Lya; Lyam; Lyam-1; Lyam1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; selectin, lymphocyte; Sell

    For Research Use Only. Not for Use in Diagnostic Procedures.
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