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MED13 (E4E9H) Rabbit Monoclonal Antibody #91684

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 230
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MED13 (E4E9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total MED13 protein. This antibody recognizes a 50 kDa protein of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1592 of human MED13 protein.

    Background

    The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10).

    Alternate Names

    Activator-recruited cofactor 250 kDa component; ARC250; DRIP250; HSPC221; KIAA0593; MED13; Mediator complex subunit 13; Mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; MRD61; THRAP1; thyroid hormone receptor associated protein 1; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; TRAP240; Vitamin D3 receptor-interacting protein complex component DRIP250

    For Research Use Only. Not for Use in Diagnostic Procedures.
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