Cat. # | Size | Qty. | Price |
---|---|---|---|
92145S | 100 µl |
|
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 135 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His34 of human TTF-I protein. Antibodies are purified by peptide affinity chromatography.
Transcription termination factor I (TTF-I) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF-I binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3’ ends of the genes. Binding of TTF-I to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC (1-5). TTF-I also has an activating function by binding an upstream terminator element to induce chromatin remodeling (6). TTF-I localization can be controlled by key tumor suppressor ARF, which binds to the TTF-I nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis (7). Furthermore, TTF-I can be ubiquitinylated by MDM2, which targets it for degradation (8). TTF-I has also been identified to play a role in HIV-1 replication (9).
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