PAXX (D6X7X) Rabbit Monoclonal Antibody #92448
- WB
- IP
Product Specifications
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
PAXX, (Paralog of XRCC4 and XLF, also known as C9orf142 or XLS), is a more recently identified component of the NHEJ machinery whose crystal structure resembles that of XRCC4 (2). PAXX directly interacts with Ku, and promotes accumulation of Ku at DSBs (2,3). Depletion of PAXX impairs cellular DSB repair (2-4,5). Paxx -/- mice develop normally with mild radiosensitivity, but a Paxx/Xlf double knockout is embryonic lethal in mice, indicating synthetic lethality between Paxx and Xlf (6). Paxx/Xlf double knockout have increased apopotosis in post-mitotic motor neurons, as well as impaired development of the adaptive immune system (7).
- Tsai, C.J. et al. (2007) Proc Natl Acad Sci U S A 104, 7851-6.
- Ochi, T. et al. (2015) Science 347, 185-188.
- Liu, X. et al. (2017) Nat Commun 8, 13816.
- Craxton, A. et al. (2015) Cell Death Differ 22, 890-7.
- Xing, M. et al. (2015) Nat Commun 6, 6233.
- Balmus, G. et al. (2016) Genes Dev 30, 2152-2157.
- Abramowski, V. et al. (2017) Cell Death Differ, 444-452.
Alternate Names
C9orf142; CI142; LOC286257; PAralog of XRCC4 and XLF; PAXX; PAXX non-homologous end joining factor; Protein PAXX; XLS; XRCC4-like small protein
Limited Uses
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