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PathScan® RP Phospho-NPM1 (Thr199) Sandwich ELISA Kit #93122

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  • ELISA

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    Product Specifications

    REACTIVITY H M R Mk
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Phospho-NPM1 (Thr199) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of NPM1 protein phosphorylated at Thr199 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-NPM1 (Thr199) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-NPM1 (Thr199). Learn more about your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP Phospho-NPM1 (Thr199) Sandwich ELISA Kit detects endogenous levels of NPM1 protein phosphorylated at Thr199. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Background

    Nucleophosmin (NPM1), also known as B23, numatrin, or NO38, is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication, and molecular chaperoning activities (1,2). The NPM1 gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM1 portion contributes to transformation by providing a dimerization domain, which activates the fused kinase (3,4).

    Alternate Names

    B23; MGC104254; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128

    For Research Use Only. Not for Use in Diagnostic Procedures.
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