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ZBTB32 (F5O1L) Rabbit Monoclonal Antibody #94103

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  • WB
  • F

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZBTB32 (F5O1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZBTB32 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse ZBTB32 protein.

    Background

    Zinc finger and BTB domain-containing 32 (ZBTB32), also known as testis zinc finger protein (TZFP) and repressor of GATA3 (ROG), is a transcription factor that belongs to the BTB/POZ-ZF protein family and was first identified in immune cell development and tumorigenesis (1,2). These transcription factor family members contain an N-terminal BTB/POZ domain, which mediates dimerization, and a C-terminal domain with a variable number of zinc fingers that bind DNA (3). ZBTB32 plays a role in T cell homeostasis and inhibits the development of Th2 cells by repressing GATA3 (4,5), and is required for the regulation of CD8+ T cells in response to viral infection (6). ZBTB32 is highly expressed by a subset of human and mouse memory B cells but not by naïve B cells (7,8), and is essential for the proliferative burst of natural killer (NK) cells in vivo (9).

    Alternate Names

    4930524C15Rik; FAXF; FAZF; GATA repressor; OTTMUSP00000026619; PLZP; Repressor of GATA; Rog; Testis zinc finger protein; Tzf; Tzfp; ZBT32; Zbtb32; zinc finger and BTB domain containing 32; Zinc finger and BTB domain-containing protein 32

    For Research Use Only. Not for Use in Diagnostic Procedures.
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