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NeuN (E4M5P) Mouse Monoclonal Antibody #94403

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 46-55
    Source/Isotype Mouse IgG2a kappa
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:400 - 1:600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #93972.

    Protocol

    Specificity / Sensitivity

    NeuN (E4M5P) Mouse Monoclonal Antibody recognizes endogenous levels of total NeuN protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa. Species cross-reactivity for immunofluorescence is mouse only.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NeuN protein.

    Background

    Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).

    Alternate Names

    FLJ56884; FLJ58356; fox-1 homolog C; Fox-3; FOX3; hCG_1776007; hexaribonucleotide binding protein 3; HRNBP3; NEUN; NeuN antigen; neuronal nuclei; Neuronal nuclei antigen; RBFOX3; RFOX3; RNA binding fox-1 homolog 3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog 3

    For Research Use Only. Not for Use in Diagnostic Procedures.
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