Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SGPP1 (F2B6W) Rabbit Monoclonal Antibody #96703

Filter:
  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:100
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SGPP1 (F2B6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SGPP1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala78 of human SGPP1 protein.

    Background

    Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator that affects various cellular functions, including immune response, neurogenesis, and angiogenesis (1). Sphingosine-1-phosphate phosphatase 1 (SGPP1) dephosphorylates S1P back to sphingosine, which is then readily converted to ceramide in the endoplasmic reticulum (ER) (2). The activity balance between SPHKs and SGPP1 is crucial for regulating sphingolipid metabolites and their downstream effects (3). SGPP1 overexpression results in elevated ceramide levels, which ultimately promotes apoptosis (4). Conversely, SGPP1 depletion increases S1P levels and induces autophagy and ER stress (5). Elevated S1P due to SGPP1 loss may also provide cellular resistance to cytotoxic agents, and SGPP1 has been found to be downregulated in gastric cancer and multiple myeloma (MM) (6-8).

    Alternate Names

    hSPP1; hSPPase1; SGPP1; Sphingosine-1-phosphatase 1; Sphingosine-1-phosphate phosphatase 1; Sphingosine-1-phosphate phosphohydrolase 1; SPP-1; Spp1; SPPase1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.