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  3. ROR gamma/ROR gamma(t) (F1S2O) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ROR gamma/ROR gamma(t) (F1S2O) Rabbit Monoclonal Antibody #96849

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  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ROR gamma/ROR gamma(t) (F1S2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total ROR gamma/ROR gamma(t) protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the N-terminus of human ROR gamma protein. This antibody recognizes residues surrounding Gln142 of human ROR gamma protein.

    Background

    RAR-related orphan receptor gamma (RORγ) is encoded by the RORC gene, and belongs to the nuclear receptor family of transcription factors (1). Two isoforms of RORγ have been described: the canonical isoform (also known as RORγ1), and a shorter isoform (known as RORγt or RORγ2). The messenger RNAs for both are transcribed from the RORC gene, but alternative exon usage yields the two distinct isoforms, which differ in the amino-terminal region. Abundant expression of mRNA encoding RORγ1 is detected in multiple tissue types, but little evidence exists to support RORy1 expression at the protein level. By contrast, expression of RORγt protein is readily detected, but appears to be restricted exclusively to distinct subsets of thymocytes and their derivatives (2). Functional studies in mice and humans have revealed that RORγt functions as a master regulator that coordinates immune cell development at multiple stages (3). For example, isoform-specific gene knockout studies in mice have shown that RORγt specifically is indispensable for fetal development of lymph nodes and Peyer's patches (3,4). Postnatally, RORγt functions as a critical regulator of Th17 cell lineage commitment, suggesting that RORγt may have potential as a therapeutic target in inflammatory diseases (5).

    Alternate Names

    IMD42; MGC129539; NR1F3; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR related orphan receptor C; RAR-related orphan nuclear receptor variant 2; RAR-related orphan receptor C; RAR-related orphan receptor C, isoform a; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; RORC; RORG; RZR-GAMMA; RZRG; TOR

    Database Links

    UniProt ID: P51449


    Entrez-Gene Id: 6097


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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