Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DGK theta (F1I5V) Rabbit Monoclonal Antibody #96927

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 101
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DGK theta (F1I5V) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGKθ protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DGKθ protein.

    Background

    Diacylglycerol kinase theta (DGKθ) is one of the mammalian diacylglycerol kinases that catalyze the conversion of diacylglycerol to phosphatidic acid (1). Hepatic sn-1,2-diacylglycerol (sn-1,2-DAG) is a second messenger that activates protein kinase Cε (PKCε). Activated PKCε is responsible for obesity-related lipid metabolism disorders that can lead to hepatic insulin resistance and type 2 diabetes. Studies show that phytochemical atractylenolide II (AT II) binds to and allosterically activates DGKθ, thereby downregulating hepatic sn-1,2-DAG levels and inhibiting PKCε activity. This results in the improvement in obesity-induced hyperlipidemia, hepatosteatosis, and insulin resistance (2). Furthermore, DGKθ-dependent activation of PPARα has a role in reducing lipid accumulation in high-fat diet-induced obese mice (3).

    Alternate Names

    DAG kinase theta; DAGK; DAGK4; DAGK7; DGK-theta; DGKQ; Diacylglycerol kinase theta; diacylglycerol kinase, theta 110kDa; Diglyceride kinase theta; testis tissue sperm-binding protein Li 38a

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.