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PBK/TOPK (F2X2Z) Rabbit Monoclonal Antibody #97728

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  • WB
  • IHC

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunohistochemistry (Paraffin) 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PBK/TOPK (F2X2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total PBK/TOPK protein. Non-specific cytoplasmic staining was observed in human and mouse skeletal muscle and in mouse spleen by immunohistochemistry.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PBK/TOPK protein.

    Background

    PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-binding domain, which is thought to interact with the human homologue of the Drosophila Discs large (hDlg) tumor suppressor protein (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclin B has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (5). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).

    Alternate Names

    Cancer/testis antigen 84; CT84; epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PBK; PDZ binding kinase; PDZ-binding kinase; serine/threonine protein kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK

    For Research Use Only. Not for Use in Diagnostic Procedures.
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