GATA-3 (D13C9) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate) #97734
Product Specifications
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 750 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #5852
Fluorescent Properties
- ← Excitation: 749 nm ← Emission: 775 nm
Product Usage Information
SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.
Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
Storage
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 null mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).
- Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
- Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
- Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
- Debacker, C. et al. (1999) Mech Dev 85, 183-7.
- Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
- Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
- Amsen, D. et al. (2007) Immunity 27, 89-99.
- Grote, D. et al. (2008) PLoS Genet 4, e1000316.
- Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
- Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
- Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
- Van Esch, H. et al. (2000) Nature 406, 419-22.
Alternate Names
GATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3
Limited Uses
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