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MAD2L2 (F9H9U) Rabbit Monoclonal Antibody #98423

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 24
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAD2L2 (F9H9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAD2L2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val203 of human MAD2L2 protein.

    Background

    Mitotic arrest deficient 2 like 2 (MAD2L2), also known as REV7, is a Mad family mitotic spindle assembly checkpoint protein that regulates progression into anaphase of mitosis (1), and functions in translesion DNA synthesis and DNA double-strand break (DSB) repair (2). MAD2L2 expression is high in human cancer, and expression levels are correlated with prognosis. MAD2L2 expression may predict the effectiveness of chemotherapy and PARP inhibitors, and MAD2L2 inhibitors may enhance the effects of these treatments (3).

    Alternate Names

    FANCV; hREV7; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2; MAD2 mitotic arrest deficient-like 2; MAD2 mitotic arrest deficient-like 2 (yeast); MAD2-like protein 2; MAD2B; MAD2L2; MD2L2; mitotic arrest deficient 2 like 2; Mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; Mitotic spindle assembly checkpoint protein MAD2B; polymerase (DNA-directed), zeta 2, accessory subunit; POLZ2; REV7; REV7 homolog

    For Research Use Only. Not for Use in Diagnostic Procedures.
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