Phospho-p90RSK (Ser380) (D5D8) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate) #98966
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 750 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #12032
Fluorescent Properties
- ← Excitation: 749 nm ← Emission: 775 nm
Product Usage Information
SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.
Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
Storage
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Upon mitogenic stimulation, p44/42 ERK1/2 and ERK5 MAP kinases cooperatively phosphorylate p90RSK Thr573 (p90RSK1 numbering) located within the C-terminal kinase domain and Thr359/Ser363 in the linker region between the two kinase domains (3). Phosphorylation of Thr573 within the activation loop of the p90RSK C-terminal kinase domain promotes activation and directs phosphorylation of Ser380 within the hydrophobic stretch of the linker region (4,5). The phosphorylated p90RSK Ser380 acts as a docking site for the constitutively active Ser/Thr kinase PDK1, which in turn phosphorylates Ser221 within the N-terminal kinase domain activation loop, resulting in full enzymatic activation of the p90RSK (6). Antibodies against these phosphorylation sites are useful for understanding the kinetics and regulation of p90RSK activation.
For more information regarding the phospho-regulatory sites within each RSK isoform, including more information regarding the seminal studies demonstrating the complex phosphorylation cascades involved, please see the references herein and PhosphoSitePlus® (www.phosphosite.org).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Roux, P.P. et al. (2003) Mol Cell Biol 23, 4796-804.
- Cargnello, M. and Roux, P.P. (2011) Microbiol Mol Biol Rev 75, 50-83.
- Romeo, Y. et al. (2012) Biochem J 441, 553-69.
Alternate Names
90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; CLS; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); epididymis secretory sperm binding protein; HU-1; HU-2; HU-3; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A1; KS6A2; KS6A3; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1c; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK-1c; MAPKAPK1; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; mental retardation, X-linked 19; mitogen-activated protein kinase-activated protein kinase 1C; MRX19; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK2; p90-RSK3; p90RSK; p90RSK1; p90RSK2; p90RSK3; p90S6K; pp90RSK2; pp90RSK3; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase A2; ribosomal protein S6 kinase A3; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; RPS6KA1; RPS6KA2; RPS6KA3; RSK; RSK-1; RSK-2; RSK-3; RSK1; RSK2; RSK3; S6K-alpha; S6K-alpha 1; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha2; S6K-alpha3
Limited Uses
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