Product Pathways - Cell Cycle / Checkpoint
Cell Cycle Regulation Antibody Sampler Kit #9932
|9932S||1 Kit (8 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|CDK2 (78B2) Rabbit mAb #2546||40 µl||W, IP, F||H, M, R, Mk||33||Rabbit IgG|
|Cyclin D1 (DCS6) Mouse mAb #2926||40 µl||W, IP||H, M, R||36||Mouse IgG2a|
|p27 Kip1 (D69C12) XP® Rabbit mAb #3686||40 µl||W, IP, IF-IC, F||H, R, Mk||27||Rabbit IgG|
|CDK6 (DCS83) Mouse mAb #3136||40 µl||W||H, M, R||36||Mouse IgG1|
|Cyclin D3 (DCS22) Mouse mAb #2936||40 µl||W, IHC-P||H, M, R||31||Mouse IgG1|
|p21 Waf1/Cip1 (12D1) Rabbit mAb #2947||40 µl||W, IP, IHC-P, IF-IC, F||H, Mk||Dg||21||Rabbit IgG|
|CDK4 (D9G3E) Rabbit mAb #12790||40 µl||W, IHC-P, IF-IC||H, Mk||30||Rabbit IgG|
|p18 INK4C (DCS118) Mouse mAb #2896||40 µl||W, IP||H||18||Mouse IgG2a|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb #12790.
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb #2546.
Western blot analysis of extracts from HeLa and NIH/3T3 cells using Cyclin D1 (DCS) mAb #2926.
Western blot analysis of extracts from SK-N-MC, C6 and IMCD3 cells using Cyclin D3 (DCS22) mAb #2936.
Western blot analysis of extracts from various cell types using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.
Western blot analysis of extracts from HeLa, IM-CD-3 and C6 cell lysates using CDK6 (DCS83) mAb #3136.
Immunohistochemical staining of cyclin D1 in paraffin-embedded human breast carcinoma showing nuclear localization, using Cyclin D1 (DCS6) mAb #2926.
Cell Cycle Regulation Antibody Sampler kit offers an economical way of detecting eight integral cell cycle regulation proteins. The kit contains enough primary and secondary antibodies to perform four Western blot experiments with each primary antibody.
Specificity / Sensitivity
Antibodies detect endogenous levels of their respective proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic peptides and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.
Eukaryotic cell cycle progression is dependent, in part, on the tightly regulated activity of cyclin dependent kinases (CDKs). Cyclin D/CDK4/6 activity occurs in mid-late G1 phase, upstream of CDK2/cyclin E activity. Both of these activities are required for hyperphosphorylation of the retinoblastoma gene product (pRb). pRb phosphorylation allows the release of S phase-promoting transcription factors and is indicative of the cell's commitment to proliferate. This point in the cell cycle is known as the restriction point. Cyclin protein levels oscillate throughout the cell cycle, and their availability is a means of controlling CDK activity and cell proliferation. Cyclin D is degraded through the ubiquitin proteasome pathway in the absence of mitogenic signaling. Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3b (GSK-3b) (1). p27/Kip1, p57 Kip2 and p21 Waf1/Cip1 are members of the Cip/Kip family of cyclin-dependent kinase inhibitors. They form heterotrimeric complexes with cyclins and CDKs, inhibiting kinase activity and blocking progression through G1/S phase (2). However, p21 may enhance assembly and activity of cyclin D/CDK4/6 complexes (3). Levels of p21 and p27 protein are controlled through ubiquitination and proteasomal degradation (4). Levels of p27 are upregulated in quiescent cells and in cells treated with negative cell cycle regulators. p27 nuclear localization is controlled by Akt-dependent phosphorylation at Thr157 (5). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C, and p19 INK4D. All INK4 proteins selectively inhibit CDK4/6 activity, either in a binary complex, or in a ternary complex including cyclin D, resulting in inhibition of cell division (6,7).
- Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.
- Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.
- Cheng, J. et al. (1999) EMBO J. 18, 1571-1573.
- Sheaff, R.J. et al. (2000) Cell 5, 403-410.
- Shin, I. et al. (2002) Nat. Med. 8, 1145-1152.
- Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
- Chang, S.F. et al. (2008) Proc Natl Acad Sci U S A 105, 3927-32. Applications: Western Blotting.
- Yamanaka, Y. et al. (2009) Blood 114, 3265-75. Applications: Western Blotting.
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* Product-specific protocol.
- 9917 Cell Cycle/Checkpoint Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2922 Cyclin D1 Antibody
- 2924 Cyclin D2 Antibody
- 2896 p18 INK4C (DCS118) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.