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HPSE (E3N3H) Rabbit Monoclonal Antibody #99756

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50, 65
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HPSE (E3N3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total HPSE protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu466 of human HPSE protein.

    Background

    Heparanase (HPSE) is an endo-β-D-endoglycosidase that catalyzes the cleavage heparan sulfate (HS) side chains from proteoglycans at the cell surface (1). The protein is synthesized as a 65 kDa inactive precursor that undergoes proteolytic processing, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme (2). HPSE serves as a key modulator of extracellular matrix (ECM) basic structure. The cleavage of HS from proteoglycans and associated changes at the ECM also cause the release of growth factors from the matrix to bind to their cognate receptors, leading to activation of downstream signaling pathways (3). A small percentage of HPSE localizes to specialized lipid raft regions. There, HPSE activates multiple signaling pathways, such as Akt, ERK, and Src pathways, by a nonenzymatic mechanism mediated by its C-terminal domain (4,5). HPSE is involved in the development of diseases such as fibrosis initiation, inflammation, tumor progression, and viral infection (6-9). HPSE inhibitors have been developed and pursued as novel therapeutics for these diseases (10-12).

    Alternate Names

    Endo-glucoronidase; HEP; Heparanase; Heparanase 50 kDa subunit; Heparanase 8 kDa subunit; heparanase exon 9&10 deletion; heparanase exon10-deletion; Heparanase-1; HPA; HPA1; HPR1; HPSE; HPSE1; HSE1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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