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35573
2’,3’-cGAMP (sodium salt)
Activators & Inhibitors
Chemical Modulators

2’,3’-cGAMP (sodium salt) #35573

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2’,3’-cGAMP (sodium salt): Image 1

Western blot analysis of extracts from THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 16 hr) and then untransfected (-) or transfected with 2’,3’-cGAMP (sodium salt) (10 μg/mL, 6 hr; +), using Phospho-STING (Ser366) (D7C3S) Rabbit mAb #19781 (upper) or STING (D2P2F) Rabbit mAb #13647 (lower).

2’,3’-cGAMP (sodium salt): Image 2

Chemical structure of 2'3'-cGAMP (sodium salt).

Product Usage Information

2’,3’-cGAMP (sodium salt) is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 500 μg of powder in 139 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, continue to store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 718.4 g/mol
Purity

>98%

Molecular Formula

C20H22N10Na2O13P2

CAS 1441190-66-4
Solubility Soluble in DMSO at 50 mg/ml or water at 10 mg/ml.

Background

2’,3’-cGAMP (sodium salt), also known as 2’,3’-cyclic-GMP-AMP, is a second messenger produced by the enzyme cGAMP synthase (cGAS) in response to cytoplasmic DNA (1,2). cGAMP binds and activates the stimulator of interferon genes (STING), a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). The binding affinity of 2’,3’-cGAMP to STING is stronger than other cyclic di-nucleotides such as c-di-GMP, 3′2′-cGAMP, 3′3′-cGAMP, and 2′2′-cGAMP with a reported Kd value of 3.79 nM compared to 1.21 μM, 1.61 μM, 1.04 μM, and 287 nM, respectively (5).

  1. Sun, L. et al. (2013) Science 339, 786-91.
  2. Wu, J. et al. (2013) Science 339, 826-30.
  3. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
  4. Zhong, B. et al. (2008) Immunity 29, 538-50.
  5. Zhang, X. et al. (2013) Mol Cell 51, 226-35.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 35573S
Product # Size Price
35573S
500 µg $ 259