Actinomycin D #15021
Product Information
Product Usage Information
Actinoymcin D is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 398.28 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 6-48 hr.
Storage
Product Description
| Molecular Weight | 1255.4 g/mol |
| Purity | >98% |
| Molecular Formula | C62H86N12O16 |
| CAS | 50-76-0 |
| Solubility | Soluble in DMSO at 50mg/ml. |
Background
Actinomycin D is an effective anti-tumor agent and the most widely studied member of the actinomycin group of antibiotics (1). The compound, isolated from soil bacteria of the Streptomyces genus, is comprised of two cyclic pentapeptides bound by a phenoxazone group (2,3). Actinomycin D inhibits mRNA transcription in mammalian cells. Actinomycin D intercalates DNA and stabilizes topoisomerase I-DNA covalent complexes, blocking RNA chain elongation by RNA polymerase and consequently inhibiting protein synthesis (1,4,5). This mechanism of action induces p53-mediated cell cycle arrest in numerous cancer cell lines (6,7) and at high concentrations can induce apoptosis (8). Additional research studies show that Akt mediates actinomycin D-induced p53 expression (9).
- Koba, M. and Konopa, J. (2005) Postepy Hig Med Dosw (Online) 59, 290-8.
- Bensaude, O. (2011) Transcription 2, 103-108.
- Lo, Y.S. et al. (2013) Nucleic Acids Res 41, 4284-94.
- Sobell, H.M. (1985) Proc Natl Acad Sci U S A 82, 5328-31.
- Trask, D.K. and Muller, M.T. (1988) Proc Natl Acad Sci U S A 85, 1417-21.
- Chang, D. et al. (1999) Cell Growth Differ 10, 155-62.
- Khan, Q.A. and Dipple, A. (2000) Carcinogenesis 21, 1611-8.
- Fraschini, A. et al. (2005) Histol Histopathol 20, 107-17.
- Chen, C.S. et al. (2014) Oncotarget 5, 693-703.
Limited Uses
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