Actinoymcin D is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 398.28 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 6-48 hr.
Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 1 week to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
|Molecular Weight||1255.4 g/mol|
|Solubility||Soluble in DMSO at 50mg/ml.|
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