Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Aphidicolin (10 μg/ml, 24 hr; +), using Thymidine Kinase 1 (E2H7Z) Rabbit mAb #28755 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Chemical structure of Aphidicolin.
Aphidicolin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 590 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 338.5 g/mol
Molecular Formula: C20H34O4
Solubility: Soluble in DMSO at 10 mg/ml or ethanol at 1 mg/ml.
Aphidicolin is a tetracyclic diterpenoid first produced by the fungus Cephalosporium aphidicola. This antibiotic is a potent inhibitor of cellular deoxyribonucleic acid (DNA) synthesis by targeting α, ε, and δ DNA polymerases in eukaryotic cells lines with little to no effect on β or γ DNA polymerases (1,2). Studies have shown that Aphidicolin specifically binds to α DNA polymerase, resulting in the formation of a pol α-DNA-aphidicolin ternary complex that blocks DNA replication. The uses for this compound include synchronized cells at the G1/S boundary and increased gene amplification (3-6).
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