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Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (100 nM, 18 hr; right), using SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) #7695 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (200 nM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb #9891 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Chemical structure of Bafilomycin A1.

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Product Usage Information

Bafilomycin A1 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 μg in 160.56 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1 μM for up to 18 hr.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

622.83 g/mol


Purity:

>98%


Molecular Formula:

C35H58O9


Isolated from Streptomyces griseus, bafilomycin A1 is a macrolide antibiotic that reversibly inhibits late phase autophagy (1,2). By inhibiting H+-ATPase, bafilomycin A1 prevents re-acidification of synaptic vesicles following exocytosis and thereby blocks fusion of autophagosomes and lysosomes (2-5). Research studies demonstrate that bafilomycin A1 can also inhibit cell proliferation (4).


1.  Werner, G. et al. (1984) J Antibiot (Tokyo) 37, 110-7.

2.  Yoshimori, T. et al. (1991) J Biol Chem 266, 17707-12.

3.  Bowman, E.J. et al. (1988) Proc Natl Acad Sci U S A 85, 7972-6.

4.  Ohkuma, S. et al. (1993) In Vitro Cell Dev Biol Anim 29A, 862-6.

5.  Yamamoto, A. et al. (1998) Cell Struct Funct 23, 33-42.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

54645
Bafilomycin A1