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Bafilomycin A1
Activators & Inhibitors
Chemical Modulators

Bafilomycin A1 #54645

Citations (0)
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (100 nM, 18 hr; right), using SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) #7695 (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (200 nM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb #9891 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chemical structure of Bafilomycin A1.

Product Usage Information

Bafilomycin A1 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 μg in 160.56 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1 μM for up to 18 hr.


Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 622.8 g/mol
Purity >98%
Molecular Formula C35H58O9
CAS 88899-55-2
Solubility Soluble in DMSO at 50mg/ml.


Isolated from Streptomyces griseus, bafilomycin A1 is a macrolide antibiotic that reversibly inhibits late phase autophagy (1,2). By inhibiting H+-ATPase, bafilomycin A1 prevents re-acidification of synaptic vesicles following exocytosis and thereby blocks fusion of autophagosomes and lysosomes (2-5). Research studies demonstrate that bafilomycin A1 can also inhibit cell proliferation (4).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 54645
Cat. # Size Qty. Price
100 µg